Trustwell HIV Ag-Ab ELISA Kit

The TRUSTwell HIV Ag-Ab ELISA Kit is a solid phase enzyme linked immunoabsorbent assay for the qualitative detection of HIV-1 P24 antigen, anti-HIV-1 including subtype O and anti-HIV-2 antibodies (including isotype IgG, IgM and IgA) in human serum or plasm. Based on double Ag/Ab-Sandwich immunoassay technique to ensure high specificity Solid microwells pre-coated with recombinant HIV-1, HIV-2 Ag and Anti-P24 Ab Liquid conjugates composed of recombinant HIV-1, HIV-2 Ag & SA conjugated with HRP Relative sensitivity: 100% and specificity: 99.9 % Comparable accuracy to the leading other ELISA kits Clear borderlines with high accuracy positive and negative controls Breakaway microwell strips Evaluated by NIB Assay time: 90 Minutes 12-months shelf life at 2-8 °C Microwells coated with anti-P24 Ab, recombinant HIV-1 and HIV-2 antigen Biotinylated P24 Ab HIV Ag positive control HIV Ag negative control HIV negative control HRP–avidin, HIV 1+2 conjugate mixture Sample diluent Wash buffer (30 x concentrate) TMB substrate A TMB substrate B Stop solution ELISA Working Sheet